Single-step Capillary Electrophoresis for Field-amplified Sample Stacking
نویسندگان
چکیده
We developed a novel single-step capillary electrophoresis (SSCE) scheme for field-amplified sample stacking (FASS) by using a hydrophilic material polymethyl methacrylate (PMMA) microchannel chip. The capillary effect was used to introduce liquids and control capillary stop valves in a partial barrier structure of the microchannel. Through the combined action of stop valves and air vents, both sample plug formation for electrophoresis and sample injection into a separation channel were successfully performed in a single step. To optimize SSCE, different stop valve structures were evaluated. Using this method, DNA ladder was concentrated 40 fold and separated well within 1 min.
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